Description

The procedure presented in this activity quickly yields
large amounts of plant pigments, making it easier to
complete than traditional solvent extractions. Students
will learn the basics of paper chromatography,
including the use of petroleum ether/acetone solvent,
and calculating the rate of flow (Rƒ) for each pigment.
Once students have completed the experiment
they will be rewarded with a vivid chromatogram
displaying chlorophyll a and b, xanthophyll, and
carotene pigments, as well as an understanding of
the different affinities for the mobile and stationary
phases. The kit includes enough materials (you
provide the spinach) for 15 setups, a teacher’s guide,
and student copymaster. Note: It is recommended
that this procedure be performed under a fume hood.
Materials Included
•15 Chromatography vials with caps
•20 Chromatography strips
•15 Disposable pipets
•15 Metric rulers
•4 Bottles, chromatography solvent
Materials Needed But Not Provided
•15 Fresh spinach leaves
•15 Coins (ribbed coins preferably)
•Pencils
•Scissors
•Forceps
•Chemical fume hood
•Gloves
•Apron