Introduction to chromatography lab activity
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Description
Students will separate a dye mixture using polar
and non-polar solvents instead of petroleum ether/
acetone solvent. They will also use a non-polar solvent
to separate out the various pigments found in leaves
and learn the function of each pigment during
photosynthesis. The kit comes with a chlorophyll
extract, so you don’t have to waste time preparing
one. It also includes materials for ten setups, teacher’s
guide, and student copymaster. The demonstration
size has enough materials for five demonstrations.
Materials Included
•1 Vial of plant pigment extract, 2 mL (chlorophyll
solution)
•1 Vial of chromatography dye mixture, 2 mL
•1 Bottle of chromatography developer II (Isopropyl
alcohol)
•1 Bottle of chromatography developer III (Isopropyl/
water)
•1 Bottle of chromatography developer IV (Methanol/
water)
•1 Capillary sample loading tubes, pkg. 50
•50 Chromatography paper strips
•30 Chromatography reaction chambers
•1 Teacher’s Guide
•1 Student Study and Analysis Sheet (Copymaster)
Materials Needed But Not Provided
•[Chromatography developer I (Water)]
Time Requirement
•30 Minutes